Instructions
The InsertSize is considered for PairedEnd-Mode:
- effectiveReadLength = ReadLength - R1_R2_Overlap
Number of reads for is calculation is reduced by:
- Unassigned Reads due to unmatched barcodes (default: 3%)
- Unspecific Reads (don’t map in the target region, default OnTargetRate: 80%, set it to 100% for WholeGenome-Sequencing)
Target Sizes of different Whole Exome Kits
| KitName | Size in Mb |
|---|---|
| Agilent SureSelect V5 | 50 |
| Agilent SureSelect V5 + UTR | 75 |
| Agilent SureSelect V6 | 60 |
| Agilent SureSelect V6 + Cosmiq | 66 |
| Agilent SureSelect V6 + UTR | 91 |
Reads per Lane on different Illumina Instruments
| InstrumentType/Mode | Reads in M |
|---|---|
| MiSeq, normal FlowCell v3 | 20 |
| MiSeq, Micro FlowCell v3 | 4 |
| MiSeq, Nano FlowCell v3 | 1 |
| NextSeq500_MidOutput | 150 |
| NextSeq500_HighOutput | 400 |
| NextSeq2000 P1 | 100 |
| NextSeq2000 P2 | 400 |
| NextSeq2000 P3 | 1200 |
| NovaSeq_SP | 650 |
| NovaSeq_S1 | 1300 |
| NovaSeq_S2 | 3300 |
| NovaSeq_S4 | 8000 |
TODO
- include Mapping Rate
